Amplified Genomic-DNA Fragments (AGFS) produced in plasmodium falciparum might be single-stranded DNA molecules released from DNA-to-DNA transcription

I published an article in 2016, the findings of which had shown that K1 CQ-resistant and HB3 CQ-sensitive isolates of Plasmodium falciparum might produce AGFs that were renamed as CAGFs because they were associated with cell cycle. The CAGFs could be induced by chloroquine (CQ) and appeared and disappeared during cell cycle progression, which suggested that CAGFs could be single-stranded DNA (ssDNA) molecules that might be produced via DNA-to-DNA transcription. There were two discovered CAGFs that were named as UB1 and UB2. I cloned and sequenced UB1 and designed two specific primers for PCR detection of UB1 in all samples that were previously detected by arbitrarily-primed PCR. Luckly. I obtained a specific PCR band from K1 2h-sample, but I couldn’t carry on the research further to check all samples with specific PCR due to some uncontrolled factors. After carefully analysing the K1 2h-sample specific PCR band and its corresponding arbitrarily-primed PCR picture, I realized that the specific PCR band in all samples could be deduced based on the bands of arbitrarily-primed PCR. The figures in this paper have shown the results of deduced UB1 specific PCR band in all samples, which might help to confirm the research findings that CAGFs or ssDNA molecules were discovered by arbitrarily-primed PCR.