In recent times, choosing a career is an extremely difficult task due to changing interest and self-knowledge. Choosing a career that is in line with one’s interest and self-knowledge are crucial in ensuring effectiveness, efficiency and productivity. The purpose of this study was to investigate the determinants of career choice among final year students of University of Education, Winneba. The study was guided by the following research objectives: To find out the extent to which interest influence career choice of final year students and to find out which ways self-knowledge influence career choice of final year students. A quantitative approach underpinned by a descriptive survey design was used. Population comprised of all final year undergraduate students of University of Education, Winneba. Stratified and proportionate random sampling techniques were employed to select a sample size of 357 for the study. Mean, standard deviation, frequencies and percentages were used in analysing the data. The findings revealed that lack of interest as well as self-knowledge negatively influenced career choice. It was recommended that parents should be encouraged by counsellors, the school authority, government and the mass media to positively guide their children with a view to making realistic career choices based on their interest. Also, Ghana Education Service should post school counsellors to all schools to provide functional career counselling services to students to enable them develop self-knowledge and appropriately with their careers.