Phytochemical study and evaluation of the antioxidant activity of fraction to the extract of Lecaniodiscus cupanioides (Sapindaceae)

Lecaniodiscus cupanioides is a medicinal plant of the Sapindaceae family, widely used in traditional medicine in Benin. In the present work we prepared the hydro ethanolic extract from the dried leaves of this plant with an extract yield of around 23%. The quantitative estimation of flavonoids and total phenols by the colorimetric method and the Folin Ciocalteux method showed that the hydro ethanolic extract is very rich in these compounds. We did the liquid-liquid extraction from the solvent of increasing polarity and obtained apolar (cyclohexane and dichloromethane) and polar (ethyl acetate and methanol) fractions. The evaluation of the antioxidant power of ethyl acetate and methanolic fractions carried out using the method of scavenging the free radical DPPH and that of the reduction of iron of FRAP revealed that the fractions of ethyl acetate and methanolic of the hydro ethanolic extract of the leaves of Lecaniodiscus cupanioides has a higher antioxidant power than that of acorbic acid.