Isolation and characterization of Incensole and DA-23-IV (Nephthenol) from ethyl acetate stem bark extract of pseudocedrela kotschyi scheinf harms (Meliaceae)

Pseudocedrela kotschyi Schweinf Harms (Meliaceae) is used in ethnomedicine to treat various disease conditions (like ulcers, sores, rheumatism, leprosy, syphilis, fevers and tumors. The dried pulverized bark material was extracted using maceration method with methanol. The methanolic extract obtained was fractionated into water, hexane and ethyl acetate fractions. Repeated Column chromatography and preparative thin-layer chromatography (TLC) of the ethyl acetate fraction yielded two pure compounds, incensole and nephthenol. The structures of the isolated compounds were elucidated using nuclear magnetic resonance spectroscopy (1D and 2D 1HNMR and 13CNMR). This is the first time report of Incensole a cembrane diterpenoid compound in the stem bark of the plant P. kotschyi..